Detection and Enumeration of Bacteria, Yeast, Viruses, and Protozoan in Foods and Freshwater

1. Survival of pathogens on surfaces and the influence of inoculating matrix on survival capabilities

Matthew J. Igo and Donald W. Schaffner

2. Enumeration of viable cells of bacteria in food and water with flow cytometry

Jossana Pereira de Sousa Guedes, and Evandro Leite de Souza

3. Evaluation of physiological characteristics of bacterial cells in foods and water with flow cytometry

Jossana Pereira de Sousa Guedes, and Evandro Leite de Souza

4. Detection of sublethally injured cells by the selective medium plating technique

Elisa Pag?n, Daniel Berdejo, Natalia Merino, and Diego Garc?a-Gonzalo, Rafael Pag?n

5. Combining culturing technique and metabarcoding to study microbiota in the meat industry

Natalia Merino, Elisa Pag?n, Daniel Berdejo, Rafael Pag?n, and Diego Garc?a-Gonzalo

6. Assessment of in vitro biofilms by plate count and crystal violet staining: is one technique enough?

Elisa Gasc?n, Natalia Merino, Elisa Pag?n, Daniel Berdejo, Rafael Pag?n, and Diego Garc?a-Gonzalo

7. Evolution assays for the isolation of mutant bacteria resistant to natural antimicrobials

Daniel Berdejo, Elisa Pag?n, Natalia Merino, and Diego Garc?a-Gonzalo, Rafael Pag?n

8. Preparing yeast suspension through serial dilution for enumeration

C?ntia Lacerda Ramos and Karina Teixeira Magalhaes-Guedes

9. Standardizing suspension of yeast for inoculation in food fermentations

Luara Aparecida Sim?es, Ang?lica Cristina de Souza, Rosane Freitas Schwan, and Disney Ribeiro Dias

10. Enumerating yeast in foods and water using the spread plating technique

Ang?lica Cristina de Souza, Luara Aparecida Sim?es, Rosane Freitas Schwan, and Disney Ribeiro Dias

11. Enumerating distinct yeast in the same food sample

Luara Aparecida Sim?es, Ang?lica Cristina de Souza, Rosane Freitas Schwan, and Disney Ribeiro Dias

12. Detection and quantification of yeast species in food samples for quality control

C?ntia Lacerda Ramos and Karina Teixeira Magalhaes-Guedes

13. Evaluation of yeast inoculated in parallel to the autochthonous microbiota in food samples

Maria Gabriela Cruz Pedrozo Miguel, Luara Aparecida Sim?es, Disney Ribeiro Dias, and Rosane Freitas Schwan

14. Double layer plaque assay technique for enumeration of viruses surrogates

Ruthchelly Tavares da Silva, Maria Mayara de Souza Grilo, Marciane Magnani, and Geany Targino de Souza Pedrosa

15. Detection of protozoan parasites on leafy greens using multiplex PCR

Minji Kim and Karen Shapiro

16. Viability of Trypanosoma cruzi in food and beverages

Rodrigo Labello Barbosa and Karen Signori Pereira

17. Detection of Giardia cysts and Cryptosporidium oocysts in edible shellfish: choosing a target

Diego Averaldo Guiguet Leal, Ta?s Rondello Bonatti, Roberta de Lima, Rodrigo Labello Barbosa, and Regina Maura Bueno Franco

18. Protocol for the detect