PCR

PCR is the most widely used technique in molecular biology. New PCR variants offering substantial benefits to existing protocols appear on a frequent basis. PCR: Methods Express describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems including:*real time qRT-PCR*rapid generation of gene targeting constructs*PCR multiplexes*PCR-based mutagenesis*identification of microdeletions and microduplications*DNA methylation analysis*forensic genetic DNA typing*genotyping*identification of mutations in single cells*whole genome amplification*diagnosis of infectious diseases*inverse PCR-based RFLPThis book is a comprehensive research guide; every chapter discusses the merits and limitations of the available approaches and then provides fully-proven protocols with hints and tips for success. PCR: Methods Express is an essential laboratory manual for researchers in all life science fields and at all levels, from postgraduate student to principal investigator.