Immune Receptors

Preface...Table of Contents...Contributing Authors...

1. Micropatterned Ligand Arrays to Investigate Spatial Regulation of Cellular Signaling Initiated by Clustered Fc ReceptorsJordan D. Mohr, Meraj Ramezani, David Holowka, and Barbara A. Baird

2. Low-Cost and Cell-Surface-Mimic Analysis of Ligand Interactions of Biotinylated Immune Receptors Using Surface Plasmon Resonance Kimiko Kuroki, Hideo Fukuhara, Takashi Tadokoro, and Katsumi Maenaka

3. Analysis of the Sub-Cellular Localization of NLRs Upon Shigella flexneri Infection Exemplified by NOD1Christine Arnold, Kornelia Ellwanger, and Thomas A. Kufer

4. Generation of VNAR Libraries from Immunized Sharks and Selection of Target-Specific Clones Helen Dooley

5. Identification of Glycan-Specific Variable Lymphocyte Receptors using Yeast Surface Display and Glycan Microarrays Tanya R. McKitrick, Melinda S. Hanes, Charles S. Rosenberg, Jamie Heimburg-Molinaro, Max D. Cooper, Brantley R. Herrin, and Richard D. Cummings

6. Cell Aggregation Assays for Homophilic Interactions between Cell Surface ProteinAidan L. Huene and Matthew L. Nicotra

7. Measuring Mutator Enzyme Activity using an E. coli-Based Colony Formation AssayMei-Chen Liu and Sebastian D. Fugmann

8. Lamprey Variable Lymphocyte Receptor Monoclonal Antibodies for Whole Cell Surface AntigensGayathri Bommakanti

9. A Role for Secreted Immune Effectors in Microbial Biofilm Formation Revealed by Simple In Vitro Assays Assunta Liberti, Brittany A. Leigh, Zachary Graham, and Larry J. Dishaw

10. Assessment of Scavenger Receptor Cysteine Rich Domain Binding to Bacteria Janice C. Telfer, Haoting Hsu, Miles Dae Tyner, and Lauren Le Page

11. Bioinformatics Approaches for Analyzing Multigene Families Encoding Immune ReceptorsKatherine M. Buckley

12. Production and Characterization of SIV-Specific CAR/CXCR5 T CellsMary S. Pampusch, Agnes Hajduczki, Gwantwa Mwakalundwa, Elizabeth Connick, Edward A. Berger, and Pamela J. Skinner

13. Engineering IgG1 Fc Domains that Activate the Complement System Chang-Han Lee and George Delidakis

14. An Imaging Flow Cytometry Protocol for Studying Immunoregulatory Receptor-Mediated Regulation of Phagocytosis Myron A. Zwozdesky, Chenjie Fei, and James L. Stafford

15. Single Cell Sequencing for EverybodyValentina Proserpio, Vanessa Falvo, Carlotta Duval, Giacomo Donati, and Salvatore Oliviero

16. Exploring the Diversity of the B-Cell Receptor Repertoire Through High-Throughput SequencingJennifer R. Hom, Deepak Tomar, and Christopher M. Tipton

17. Fetal Thymic Organ Culture (FTOC) Optimized for Gamma-Delta T Cell StudiesJohanna S. Selvaratnam, Tracy S.H. In, and Michele K. Anderson

Subject Index List...